| I&T Solution |
On-site Rapid Testing with Centrifugal Microfluidics for Legionella
(REF: S-1995) |
| Trial Project |
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| Solution Feature |
- Legionella DNA is detected via loop-mediated isothermal amplification (LAMP) with color readouts that are discernable by eye or using a camera
- Centrifugal microfluidics are used to largely automate testing, with a hands-on sample preparation time of only ~5 minutes
- Results are obtained in 20–40 minutes, depending on Legionella abundance, with integrated triplicate testing and positive/negative controls used to enhance accuracy
- Each assay can distinguish between high (>1,000 CFU/mL) and low (10 – 1,000 CFU/mL) Legionella thresholds
- Multiple water samples (~5) can be tested on-site simultaneously, enhancing throughput and decreasing workloads
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| Trial Application and Expected Outcome |
- At least three candidate LAMP primer sets will be assessed using synthetic Legionella DNA controls. The set with the fastest time-to-result and highest sensitivity will be selected. We expect a sensitivity of at least 50 CFU per reaction and time-to-results of 15–35 minutes depending on control DNA quantity.
- We will evaluate our sample preconcentration method using water spiked with various amounts of Legionella. Preconcentration involves filtering 100–200 mL of sample through a 0.44 micron filter, passing a wash buffer, backflushing the filter with extraction buffer and ensuring extracts yield comparable LAMP outcomes as with DNA controls.
- LAMP reagents will be integrated into centrifugal microfluidic chips via lyophilization, for improved shelf-stability and user-friendliness; on-chip LAMP performance will be compared against the previous experiments conducted in microtube format. We expect comparable sensitivity and rapidity and can modify chip designs to accommodate greater sample volumes if necessary.
- We will assess our microfluidic chips with real-world water samples and compare our results against those obtained through HOKLAS accredited laboratories and quantify agreement. Laboratory results will serve as the gold standard. We expect our assay to have similar performance to those which comply with ISO 11731.
- The shelf-stability of our microfluidic chips will be assessed. LAMP experiments will be conducted using control DNA fragments and real-world water samples and compared to our prior chip and lab results. We expect a shelf-life at room temperature of at least 3 months.
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| Additional Solution Information |
Suarez2023_MicrofluidicsPrototypingVDIMMultiplexDiagnostics_Article.pdf
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| Info on I&T Solution Provider |
| Solution Provider | : | VisionDrop AI Limited | | Address | : | Unit 707, 7/F, Building 19W, No. 19 Science Park West Avenue, Hong Kong Science Park, Pak Shek Kok, N.T. | | Contact Person | : | Tang Yu Kiu Yuki |
| Position | : | Chief Operating Officer | | Tel | : | 62599527 | | Email | : |
ytang@visiondropai.com | | Webpage | : | https://visiondropai.com/ |
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